Frozen protein picture

Flash-Cooling:
A Practical Guide

From Gitay Kryger's thesis
Html-ized by Dan Peisach

Rosenstiel Basic Medical Sciences Research Center,
Brandeis University, Waltham, MA. 02254-9110

Last updated 10/18/95

INTRODUCTION
Background
Crystal Decay
Flash Cooling
METHODS
Choice of a Suitable Crystal
Choice of a Cryo-protectant
Solvent Exchange
Choice of a Mounting Assembly
Nylon Loop Construction
Materials for Loop Construction
Flash Cooling Technique and Transfer to the Camera
LOW TEMPERATURE DEVICE
Gas Flow Supply
The Cryostat
The nozzle
The Heated Goniometer Head
CONCLUDING REMARKS
REFERENCES

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Comments and feedback to Daniel Peisach