1.2 Low-temperature Device

During the course of the ASA-DH project (see chapter 1) the need to stabilize the crystals in the X-ray beam became essential to obtain data of good quality. A low temperature device, or cryostat, was constructed from an old MSC/AFC diffractometer cryostat. The principle of the device is to cool nitrogen gas to cryo-temperature by passing it through a copper coil immersed in liquid nitrogen, the heat exchange unit, and blow it at a steady rate onto the crystal in the X-ray camera. The following is a description of this machine which is now in use for all the projects requiring cryo-temperature data collection at Brandeis University.

1.2.1 Gas Flow Supply

The supply of gaseous nitrogen is a set of two high pressure, 240 liter liquid nitrogen containers (New England Air and Gas, NILG 240). One is used to draw gas while the other is a backup reserve. A pressure-sensitive automated device (see appendix C, C.1, for the automatic switch logic-circuit) switches the withdrawal from one tank to the other when the pressure in the first tank drops, an indication that the liquid nitrogen in it is below working pressure(fig 1.9).

Figure 1.9: Gas supply to cryostat

1.2.2 The Cryostat

The gas coming from the automatic switching device enters a box that splits it to three exits:
  1. The cold flow: leads to the heat exchange unit and from there to the transfer-tube through the nozzle and onto the crystal.
  2. The warm flow: leads to the nozzle and feeds the engulfing stream or dry shroud (see nozzle fig. 1.11).
  3. The auxiliary flow: leads to the transfer-tube T-section and feeds the flow to the crystal if higher temperatures are desired.

Figure 1.10: The cryostat

1.2.3 The nozzle

The nozzle is design to prevent moisture from condensation on and around the crystal. The cryo-stream flows inside a room-temperature, dry nitrogen sleeve engulfing it all around. The two co-axial flows have an effective distance of 3cm at optimal flow.

Figure 1.11: The nozzle, cryo stream and dry shroud

1.2.4 The Heated Goniometer Head

The cryo-stream is most efficient when pointing down. It is important to prevent the goniometer head from freezing, an event that might lead to x-y translation-slides and spindle arrest. This is afforded by inserting a thin flexible Kapton heater (Omegalux(TM) model KHLV 0502/10 by Omega) under the top translation-slide in the basic Charles Supper goniometer head (fig 1.12). Figure C.6 in appendix C shows the heated goniometer head in action during IPM-DH data collection.

Figure 1.12: A basic Charles Supper goniometer head modified
to be used for cryo-crystallography by the addition of a heater
Figure 1.13: The final arrangement of the cryostat nozzle
in use over the heated goniometer head.

1.2.5 Automatic Liquid Nitrogen Refill Device

To minimize the need for human intervention to once in every two days, the system is fitted with a liquid nitrogen level controller (Gordinier Electronics and Cryogenics, model LL459). This device will maintain the level of liquid nitrogen in the cryostat container within a set range and will sound an alarm if the level is over or below high and low limits respectively (fig. 1.10).
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